sCircle®
- Full-length immune repertoire profiling
The sCircle® Single Cell Full Length Immunoreceptor Library Kit technology enables full length sequencing of the V(D)J region at single cell level with short-read sequencing for seamless precision in immune profiling.
Kit Highlights
Demo Data
Figure: Receptor Pairing Map
sCircle® Single Cell Full Length Immunoreceptor Library Kit has significant advantages compared to other methods due to the circularization process of the immunoreceptor cDNA specifically designed to reverse the sequence of the transcript and, through this, allows the full-length of the immune receptor to be sequenced. This results in reads mapped to the full-length sequence of the immunoreceptor gene (BCR or TCR), including the V(D)J region and part of the constant region. It also shows a high immunoreceptor pairing rate, and a high number of immune receptor transcript-specific UMIs detected per cell, rendering both high specificity and sensitivity.