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Dissociated biopsies from lung cancer patients analyzed by flow cytometry

Figure A:

TME cell composition can greatly influence cancer prognosis and the choice of therapy. Here, cancerous tissue from a patient with Lung Adenocarcinoma (LUAD) was extracted and dissociated using sCelLiVE® (flow) in combination with PythoN®, stained with antibodies (n=21), pooled, and further analysed with FACS. The specific cell type can be determined by studying the co-expression of surface markers. Among the non-WBC analysed, it was observed how EpCAM, a tumour marker for cancer cells of epithelial origin, is frequently co-expressed with PD-L1 on, advocating for anti-PD-L1 therapy. 

Dissociated biopsies from lung cancer patients analyzed by flow cytometry

Figure B:

(1) Once knowing the cell composition, and the proportion between epithelial cell and T-cells, one can categorize the tumour into cold tumour (less immune cell, hence poor response to immunotherapy) and hot tumour.
(2) By further investigating the cell types between patients with different types of lung cancer, Lung Squamous-cell Carcinoma (LUSC) seem to have a significantly high tissue (epithelial cells) ratio, advocating for a different strategy than immunotherapy.
(3) In parallel, one can investigate the T-cell population, to get more insights on the specific identity and their possible role in tumour control or immune evasion.

sCelLiVE® Flow Tissue Dissociation Kit Results

Streamlined workflow: from sample to library within one workday

From samples to library within one workday

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