During the DynaSCOPE workflow, a nucleotide analogue (uracil-analogue) is incorporated into freshly synthesized transcripts during transcription, but not into static transcripts. The incorporated uracil-analogue is converted into cytosine analogue and later recognized as cytosine (C), while the unlabeled uracil in stable transcripts is converted into thymine (T). Nascent and long-lasting transcripts can then be differentiated by bioinformatic analysis provided by the CeleScope® software.